Proteomics: Methods Express identifies the most powerful new technologies and presents them in a way that allows their robust implementation. The focus is on proteomic methods and strategies that are reliable and of general applicability. Each chapter presents descriptions of what can, and cannot, be achieved with the relevant procedures so that readers can make informed judgments prior to establishing the methods in-house. Every chapter discusses the merits and limitations of various approaches then provides tried-and-tested protocols with hints and tips for success and troubleshooting for when things go wrong.
1. Sample preparation and subcellular fractionation approaches: purification of membranes and their microdomains for mass spectrometry analysis Yan Li , Phil Oh, and Jan E. Schnitzer, Sidney Kimmel Cancer Center 2. An isotope-coding strategy for quantitative proteomics Xian Chen, Dept of Biochemistry and Biophysics, University of N. Carolina at Chapel Hill 3. Gel-based approaches Stuart Cordwell and Ben Crossett, both at School of Molecular and Microbial Biosciences, University of Sydney, and Melanie Y. White, Minomic Pty Ltd 4. Peptide sorting by reverse-phase diagonal chromatography Kris Gevaert and Joel Vandekerckhove, Faculty of Medicine and Health Sciences, Ghent University 5. Mass spectrometry strategies for protein identification David R. Goodlett, University of Washington and Garry L. Corthals, University of Turku 6. Desorption electrospray ionization: proteomics studies by a method that bridges ESI and MALDI Zoltan Takats, Justin M. Wiseman, Demian R. Ifa and R.Graham Cooks, all at Dept of Chemistry, Purdue University 7. Analysis of cellular protein complexes by affinity purification and mass spectrometry Tilmann Buerckstuemmer and Keiryn L. Bennett, both at Research Center for Molecular Medicine of the Austrian Academy of Sciences 8. Clinical proteomic profiling and disease signatures Rosamonde E. Banks, David A. Cairns, David N. Perkins and Jennifer H. Barrett, all at Cancer Research UK Clinical Centre, St James' s University Hospital, Leeds 9. Characterization of post-translational modifications: undertaking the phosphoproteome W. Andy Tao, Purdue University; Bernd Bodenmiller and Ruedi Aebersold, both Institute for Molecular Systems Biology, Federal Institute of Technology, Zurich 10. Protein microarray technologies Chien-Sheng Chen, Sheng-Ce Tao, and Heng Zhu, Dept of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine 11. Intelligent mining of complex data: challenging the proteomic bottleneck Dan Bach Kristensen, Maxygen and Alexandre Potelejnikov, Proxeon 12. Bioinformatic approaches in proteomics Sandra Orchard and Henning Hermjakob, both European Bioinformatics Institute, Hinxton List of suppliers Index
