Laboratory Manual for Exercise Physiology

G. Gregory Haff, PhD, CSCS,*D, FNSCA, is a full professor and the course coordinator for the postgraduate degree in strength and conditioning at Edith Cowan University in Joondalup, Australia. Haff has published more than 150 articles, centering his research on performance effects in the areas of strength training, cycling, and nutritional supplementation. Haff is a past president of the National Strength and Conditioning Association (NSCA) and a senior associate editor for the Journal of Strength and Conditioning Research. He was the United Kingdom Strength and Conditioning Association (UKSCA) Strength and Conditioning Coach of the Year for Education and Research and the 2011 NSCA William J. Kraemer Outstanding Sport Scientist Award winner. He is a certified strength and conditioning specialist with distinction (CSCS,*D), a UKSCA-accredited strength and conditioning coach (ASCC), and an accredited Australian Strength and Conditioning Association level 2 strength and conditioning coach. Additionally, Haff is a national-level weightlifting coach in the United States and Australia. He serves as a consultant for numerous sporting bodies, including teams in the Australian Football League, Australian Rugby Union, Australian Basketball Association, and National Football League. Charles Dumke, PhD, is a full professor and graduate program coordinator in the School of Integrative Physiology and Athletic Training at the University of Montana. He has taught both undergraduate and graduate courses in exercise physiology and sport nutrition for over 20 years. He earned his doctoral degree in kinesiology from the University of Wisconsin at Madison. His areas of interest in exercise science are energy expenditure, fuel utilization, economy of movement, mechanisms of mitochondrial adaptation, and diabetes. He has published more than 120 peer-reviewed articles. In his free time, Dumke enjoys competing in triathlons, biking, running, taking on building projects with little know-how, and coaching his son in all sorts of sports.

Laboratory 1. Primary Data Collection Test Variables Measurement Terminology Metric Conversions Background and Environmental Information Descriptive Statistics Presentation of Results Interpretation of Data Poster Presentations Laboratory Activity 1.1 Basic Data Laboratory Activity 1.2 Statistical Procedures Laboratory Activity 1.3 Tables and Graphs Laboratory Activity 1.4 Creating a Poster Presentation Laboratory 2. Pretest Screening Informed Consent Physical Activity Readiness Questionnaire for Everyone Health History Questionnaire Signs and Symptoms of Disease and Medical Clearance Coronary Risk Factor Analysis Lifestyle Evaluation Disease Risk Stratification Laboratory Activity 2.1 Basic Screening Procedures Laboratory Activity 2.2 Pretest Results Laboratory 3. Flexibility Testing Direct and Indirect ROM Assessment Body Areas Beighton Scoring System Laboratory Activity 3.1 Traditional, Wall, V-Sit, and Chair Sit-and-Reach Test Comparisons Laboratory Activity 3.2 YMCA, Backsaver, and Goniometer Test Comparisons Laboratory Activity 3.3 Canadian, Traditional, and Backsaver Sit-and-Reach Test Comparisons Laboratory Activity 3.4 Shoulder Flexibility Test Comparisons Laboratory Activity 3.5 Measuring Ankle Dorsiflexion Range of Motion (ROM) Laboratory Activity 3.6 Beighton Scoring System Test Laboratory 4. Blood Pressure Measurements Blood Pressure Responses to Exercise Accurate Blood Pressure Checks Blood Pressure Devices Laboratory Activity 4.1 Effects of Body Position on Blood Pressure Laboratory Activity 4.2 Effects of Dynamic Exercise on Blood Pressure Laboratory Activity 4.3 Effects of Isometric Contractions on Blood Pressure Laboratory 5. Resting Metabolic Rate Determinations Aerobic Metabolism and Respiratory Exchange Ratio Total Energy Expenditure and Resting Metabolic Rate (RMR) Laboratory Activity 5.1 Estimating Resting Metabolic Rate (RMR) Laboratory Activity 5.2 Measuring Resting Metabolic Rate (RMR) Laboratory 6. Oxygen Deficit and EPOC Evaluations Transition From Rest to Exercise Oxygen Uptake During Exercise and Recovery Laboratory Activity 6.1 Calculation of Oxygen Deficit and EPOC on a Treadmill Laboratory Activity 6.2 Calculation of Oxygen Deficit and EPOC on a Bike Laboratory Activity 6.3 EPOC Following a Wingate Test Laboratory 7. Submaximal Exercise Testing Measurement of Heart Rate Rating of Perceived Exertion Laboratory Activity 7.1 Submaximal Bench Step Test Laboratory Activity 7.2 Submaximal Treadmill Test Laboratory Activity 7.3 Submaximal Cycle Ergometer Test Laboratory 8. Aerobic Power Field Assessments Cooper 1.5-Mile Run/Walk Test Cooper 12-Minute Run/Walk Test Rockport Fitness Walking Test 6-Minute Walk Test (6MWT) Laboratory Activity 8.1 Cooper 1.5-Mile Run/Walk Test and 12-Minute Run/Walk Test Laboratory Activity 8.2 Rockport Fitness Walking Test Laboratory Activity 8.3 6-Minute Walking Test (6MWT) Laboratory 9. High-Intensity Fitness Testing Leger 20 m Shuttle Run Test Yo-Yo Intermittent Recovery Test 30-15 Intermittent Fitness Test Laboratory Activity 9.1 Leger 20 m Shuttle Run Test (20mSRT) Laboratory Activity 9.2 Yo-Yo Intermittent Recovery Test (Yo-Yo IRT) Laboratory Activity 9.3 30-15 Intermittent Fitness Test (30-15IFT-40m) Laboratory Activity 9.4 Modified 30-15 Intermittent Fitness Test (30-15IFT-28m) Laboratory 10. Maximal Oxygen Consumption Measurements Selecting a Test Protocol Monitoring Progress With RPE Scales Estimating Fuel Usage With RER Ventilatory Threshold Laboratory Activity 10.1 Graded Treadmill VO2max Test Laboratory Activity 10.2 Cycle Ergometer VO2max Test Laboratory 11. Blood Lactate Threshold Assessment Determining the Deflection Point Selecting a Test Method Role of the Ventilatory Threshold Laboratory Activity 11.1 Blood Lactate Measurement at Rest Laboratory Activity 11.2 LT During an Incremental Cycle Test Laboratory Activity 11.3 Blood Lactate After Anaerobic Exercise Laboratory 12. Musculoskeletal Fitness Measurements Assessments of Muscular Strength Assessments of Muscular Endurance Laboratory Activity 12.1 Maximal Upper-Body Strength Laboratory Activity 12.2 Creating a Load-Velocity Profile and Predicting 1RM Laboratory Activity 12.3 Maximal Lower-Body Strength Laboratory Activity 12.4 Maximal Handgrip Strength Laboratory Activity 12.5 Upper-Body Muscular Endurance Laboratory 13. Anaerobic Fitness Measurements Sprinting Performance Tests for Estimating Horizontal Power Jumping Performance Tests for Determining Vertical Power Formulas for Estimating Vertical Power Testing Interlimb Asymmetries With Bilateral and Unilateral Jump Tests Bosco Test for Estimating Power Endurance Determining the Eccentric Utilization Ratio Wingate Anaerobic Test for Determining Anaerobic Cycling Power Margaria-Kalamen Stair-Climb Test for Determining Anaerobic Power Laboratory Activity 13.1 Sprinting Performance Laboratory Activity 13.2 Jumping Performance Laboratory Activity 13.3 Jumping Performance With a Switch Mat Laboratory Activity 13.4 Jump Performance: Comparing a Smartphone App With a Switch Mat Laboratory Activity 13.5 Determining Asymmetries With a Force Plate Laboratory Activity 13.6 Power Endurance Laboratory Activity 13.7 Anaerobic Cycling Power Laboratory Activity 13.8 Margaria-Kalamen Stair-Climb Test Laboratory 14. Pulmonary Function Testing Pulmonary Function Testing Pulmonary Function Testing as a Tool for Diagnosing Pulmonary Disease Respiratory Limitations on Exercise Laboratory Activity 14.1 Lung Volumes and Capacities Laboratory Activity 14.2 Pulmonary Function Laboratory Activity 14.3 Exercise-Induced Ventilatory Limitations Laboratory Activity 14.4 Exercise-Induced Asthma Laboratory 15. Body Composition Assessments Body Composition Models BMI for Categorizing Health Risk Circumference Measurements and Health Risk Skinfold Thickness as a Measure of Body Fat Laboratory Activity 15.1 BMI and Circumference Data Laboratory Activity 15.2 Bioelectrical Impedance Laboratory Activity 15.3 Techniques for Measuring Skinfold Thickness Laboratory Activity 15.4 Estimating Relative Body Fat Using Hydrodensitometry Laboratory 16. Electrocardiograph Measurements Electrical Activity of the Heart Placement of ECG Leads Interpreting the ECG Recording ECG as a Tool for Diagnosing Cardiac Abnormalities Heart Rate Response to Exercise Laboratory Activity 16.1 Resting ECG Laboratory Activity 16.2 Effects of Body Position on the Heart Axis Laboratory Activity 16.3 Submaximal Exercise Effects With a 12-Lead ECG Appendix A: Units of Measure Conversions Appendix B: Estimation of the O2 Cost of Walking, Running, and Leg Ergometry Appendix C: Haldane Transformation Appendix D: Metabolic Cart Information Appendix E: Calibration of Equipment